《植物生理学报》 2015, 51(5): 661-667

虎杖提取物对黄瓜防御酶系的影响

王远遐^1,2, 姬兰柱^1,*, 刘艳¹, 易雪梅^1,2, 张悦^1,2

¹中国科学院沈阳应用生态研究所, 沈阳110016, ²中国科学院大学, 北京100049

通信作者：姬兰柱；E-mail: ji.lanzhu@iae.ac.cn；Tel: 024-83970302

摘　要：

本文采用热回流法提取虎杖乙醇提取物, 测定不同浓度虎杖提取物处理下，黄瓜幼苗叶片中过氧化物酶(POD)、多酚氧化酶(PPO)、苯丙氨酸解氨酶(PAL)、几丁质酶(CHT)和β-1,3-葡聚糖酶(GLU)等活性的变化。结果表明, 40 mg•L^-1虎杖提取物处理下, 黄瓜叶片POD和GLU (2~5 d)活性明显高于对照, PPO和CHT活性呈现M型变化趋势, 且PPO、PAL和CHT活性与对照相比变化无规律。400 mg•L^-1虎杖提取物处理的黄瓜幼苗叶片的POD、PPO和CHT活性明显高于40 mg•L^-1的, 但也一定程度上降低了GLU活性。

关键词：虎杖; 黄瓜; 防御酶系

收稿：2014-07-07   修定：2014-11-24

资助：辽宁省科技计划项目(2012214001)和沈阳大学重点实验室基金项目(SYU-KF-L-05)。

Effect of Extract of Polygonum cuspidatum on Defensive Enzymes of Cucumis sativus

WANG Yuan-Xia^1,2, JI Lan-Zhu^1,*, LIU Yan¹, YI Xue-Mei^1,2, ZHANG Yue^1,2

¹Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China; ²University of Chinese Academy of Sciences, Beijing 100049, China

Corresponding author: JI Lan-Zhu; E-mail: ji.lanzhu@iae.ac.cn; Tel: 024-83970302

Abstract:

The ethanol extract was obtained from Polygonum cuspidatum by hot reflux extraction. The activities of peroxidase (POD), polyphenol oxidase (PPO), phenylalanine ammonialyase (PAL), chitinase (CHT) and β-1,3-glucanase (Glu) in leaves of Cucumis sativus seedling were measured under different concentrations of extract of P. cuspidatum. The results showed that under the treatment of 40 mg•L^-1 extract, the activities of POD and GLU (2–5 d) of cucumber leaves were higher than those of control. And the activities of PPO and CHT showed a trend of M. Compared with the control, the activities of PPO, PAL and CHT were irregular. Under the treatment of 400 mg•L^-1 extract, the activities of POD, PPO and CHT were obviously higher than those of 40 mg•L^-1 extract, but the activity of GLU was decreased at a certain extent.

Key words: Polygonum cuspidatum; Cucumis sativus; defensive enzymes